We recommend the use of these two biochemical and cytological changes in intestines as specific biomarkers to indicate current and recent exposure of fish to BaP via oral intake.Įthoxyresorufin- O-deethylase ( EROD) activity in fish as a biomarker of chemical exposure These results further corroborate our earlier findings that induction of EROD activities in fish do not merely indicate exposure to BaP but also are correlated to harmful biological effects.
Importantly, intestinal EROD activity was significantly correlated to 2 degrees/3 degrees lysosome accumulation in enterocytes (r = 0.571, p < 0.001). Significant increase of 2 degrees/3 degrees lysosomes, as measured by Vv(lysosome, mucosa), was detected in young enterocytes of fish in the high-dosing group (12.5 microg/g fish/d) at exposure day 3 and persisted until recovery week 2. Conversely, hepatic EROD induction was weak and subsided rapidly in the exposed fish, signifying that hepatic EROD activity is not a good indicator of oral intake of BaP.
A dose-response relationship was demonstrated between intestinal EROD activities and the levels of foodborne BaP. Intestinal EROD induction was rapid (within 3 d) and sustained in the BaP-exposed fish, while a fast recovery (within one week) was observed on withdrawal of BaP intake. Temporal changes of intestinal and hepatic ethoxyresorufin- O-deethylase ( EROD) activities and quantitative changes of secondary and tertiary (e.g., 2 degrees/3 degrees) lysosomes in enterocytes were compared for the juvenile grouper (Epinephelus coioides) on chronic exposure to foodborne benzopyrene (BaP) at two environmentally realistic levels (0.25 and 12.5 microg/g fish/d) over a four-week exposure and four-week depuration period. Temporal changes of ethoxyresorufin- o-deethylase ( EROD) activities and lysosome accumulation in intestine of fish on chronic exposure to dietary benzopyrene: linking erod induction to cytological effects. TEMPORAL TRENDS IN ETHOXYRESORUFIN- O-DEETHYLASE ACTIVITY OF BROOK TROUT (SALVELINUS FONTINALIS) FED 2,3,7,8-TETRACHLORODIBENZO-P-DIOXINĬhanges in ethoxyresorufin- O-deethylase ( EROD) activity were monitored through an extended 6-month dietary exposure to determine the relationship between EROD activity and uptake of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in brook trout, Salvelinus fontinalis.
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